Table 1. Candidates identified by ERC and tested for effects on 4-day remating receptivity.
Gene Name | Predicted functional class | Expression pattern* | Significant ERC results | Amount of knockdown | 4-Day Receptivity Assay |
CG30433 | C-type lectin | male AG | CG17575: p = 0.025CG1652: p = 0.037SP: p = 0.042 | near-complete | KD: 7/33cont: 3/30p = 0.31 |
CG11037 | chymotrypsin-like | male AG | CG9997: p = 0.015CG1652: p = 0.029 | partial | KD: 1/30cont: 0/29p = 1.00 |
CG11977 | CRISP | male AG | CG9997: p = 0.011CG1652: p = 0.049 | near-complete | KD: 2/36cont: 3/39p = 1.00 |
CG14034 | lipase | male AG | CG1652: p = 0.029CG9997: p = 0.043 | near-complete | KD: 9/30cont: 8/45p = 0.27 |
CG14061 (aqrs) | serine protease homolog | male AG | CG1652: p = 0.0015CG9997: p = 0.02CG1656: p = 0.035 | near-complete | KD: 27/29 cont: 0/29 p<0.0001 |
CG2975 | galactosyltransferase | male AG, crop | CG17575: p = 0.003SP: p = 0.03 | complete | KD: 0/34cont: 5/28p = 0.015 |
CG30488 (antr) | CRISP | male AG | CG9997: p = 0.009 | complete | KD: 29/32 cont: 3/29 p<0.0001 |
CG42326 | unknown | male AG, head, eye | CG9997: p = 0.015CG1652: p = 0.033 | near-complete | KD: 2/33cont: 3/31p = 0.67 |
CG12558 (intr) | serine protease homolog | male AG | CG9997: p = 0.007 | near-complete | KD: 11/14 cont: 3/16 p = 0.0027 |
CG42564 | CRISP | male AG | CG9997: p = 0.003 | near-complete | KD: 4/32cont: 2/33p = 0.43 |
CG8420 | unknown | male AG | CG1652: p = 0.007 | partial | KD: 1/33cont: 2/33p = 1.00 |
CG13077 | cytochrome b561 | female ST, eye, head | CG1656: p = 0.009 | near-complete | KD: 3/33cont: 9/33p = 0.11 |
CG16713 | Kunitz protease inhibitor | female ST, FB, hindgut, head, eye | CG1652: p = 0.009CG17575: p = 0.022CG9997: p = 0.042 | none detected | n/a |
CG3097 | peptidase M14 | female ST, hindgut, crop | CG9997: p = 0.0007CG1652: p = 0.011 | complete | KD: 1/36cont: 2/39p = 1.00 |
CG3239 (frma) | protease/neprilysin | female ST, FB, head, heart | CG17575: p = 0.008 | partial | KD: 21/30 cont: 1/30 p<0.0001 |
CG4302 | UDP-glucosyltransferase | female ST, MT, FB, eye, TG, head, brain | CG1656: p = 0.002CG9997: p = 0.021 | none detected | n/a |
CG6910 | inositol oxygenase | female ST, heart, FB | CG1656: p = 0.007CG17575: p = 0.047 | partial | KD: 1/30cont: 4/31p = 0.35 |
CG8586 | chymotrypsin-like | female ST, head, FB, eye, crop, heart | CG1656: p = 0.008SP: p = 0.022CG17575: p = 0.042 | none detected | n/a |
Mtp | phosphatidylcholine transpoter | female ST, FB, head, heart, eye, brain, TG, crop | CG1652: p = 0.041CG9997: p = 0.048 | none detected | n/a |
vkg | extracellular matrix component | female ST, FB, heart, TG, brain, head | CG17575: p = 0.007 | none detected | n/a |
CG5630 (hdly) | unknown | female ST, SG, crop, tubule, hindgut, midgut | CG17575: p = 0.005 | near-complete | KD: 15/27 cont: 3/31 p = 0.0002 |
Expression based on data from FlyAtlas [64]. Predicted functions are from FlyBase electronic annotations. Bold indicates statistical significance for positive candidates. Abbreviations are as follows: AG = accessory gland; ST = spermatheca; FB = fat body; TG = thoracicoabdominal ganglion. For examples of near-complete and partial knockdown, see Figure S7. KD: knockdown, cont: control. The 4-day recepetivity assay column shows the number of females remating out of the total number of females tested for each condition; p-values are from Fisher's exact tests.