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. 2014 Jan 16;10(1):e1004100. doi: 10.1371/journal.pgen.1004100

Figure 1. SKN-1 expression and WDR-23 sub-cellular localization in motor neurons.

Figure 1

A) Schematic of bec-1 and skn-1 genetic loci. Locations of gfp tags used in this study indicated. B) Co-localization of nls-gfp driven by the 7.3 kb Pbec-1 promoter (vjEx254) with Punc17::rfp (nuIs321) in cholinergic motor neurons. Arrowheads indicate cholinergic motor neurons; arrow indicates GABAergic neuron. C–D) Left, Localization of WDR-23a::GFP (vjEx423; C) and WDR-23b::GFP (vjEx426; D) in ventral cord motor neurons expressed under the snb-1 promoter fragment. Neuronal cell bodies are identified by the unc-17 promoter driving rfp (nuIs321) for reference. Right, WDR-23a::GFP (vjIs26; C) and WDR-23b::GFP (vjEx7; D) co-localization with the synaptic vesicle associated protein mCherry::SNB-1 (vjEx339) in motor neuron axons using the unc-129 promoter fragment. E) WDR-23a::GFP (vjIs26) co-localization with the mitochondrial marker (INVOM::RFP; vjEx663) in motor neuron axons in wild type, unc-104/kinesin mutants (E′) and drp-1/Drp1 mutants (E″). Scale bars represent 10 µm.