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. 2014 Jan 16;9(1):e83879. doi: 10.1371/journal.pone.0083879

Figure 1. Mice display stable behavior in a repeated FST paradigm.

Figure 1

(A) Outline of the repeated FST protocol performed over several days. C57BL/6N mice (n = 12) were injected at time 0 with saline (arrow) and every mouse was subjected to FST 30 min, 24 h, 48 h, 72 h and 96 h later. After 4 days of rest in their home cages mice were retested in the FST once again (i.e. 240 h after saline injection). (B) Results (sec/4 min) are presented as mean values ±SEM. Immobility time is stable after repeated FST: 161±15 (30 min); 173±17 (24 h); 199±12 (48 h); 208±9 (72 h); 186±9 (96 h) and 183±9 (240 h) (p>0.05, one way ANOVA).