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. 2014 Jan 16;9(1):e78644. doi: 10.1371/journal.pone.0078644

Figure 10. Inaccurate annotation of probe set 224321_PM_at resulted in conflicting results between Affymetrix microarray and RNA-Seq approaches for TMEFF2 expression.

Figure 10

In Affymetrix microarray studies, probe set 224321_PM_at showed high expression of TMEFF2 at 0, 2, 4 and 6 hours. However, the high expression reported by probe set 224321_PM_at is supported neither by 233910_PM_at nor by 223557_PM_s_at. In contrast, there was no detectable expression of TMEFF2 in RNA-Seq studies. As a matter of fact, the probe set 224321_PM_at is mapped to a genomic region that is unrelated to TMEFF2, and thus this Affymetrix probe set is inaccurately annotated.