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. 2014 Jan 16;9(1):e86118. doi: 10.1371/journal.pone.0086118

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© 2014 Wong et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–C, HSP47 is increased during ESC-SMC differentiation. Murine ESC were differentiated into SMC in the presence of Collagen IV for the time indicated, followed by quantitative RT-PCR analysis of HSP47 mRNA (A), immunofluorescent staining with HSP47 (green) and SM-22α (red) antibodies (B), calponin (red) and SM-αA (red) (C). DAPI was included to counterstain the nucleus (blue). Scale bar, 30 µm. Images shown are representative of at least three separate experiments, whereas graphs are shown as mean ± SEM of at least three independent experiments, ***P<0.005 compared with Day 0.