Figure 2. RDR6 negatively regulates PTI responses.

(A) H₂O₂-dependent luminescence upon H₂O or flg22 (100 nM) treatment in WT and rdr6-15 leaf discs. (B) Expression levels of PAMP-responsive mRNAs, FRK1, WRKY22 and WRKY29 detected by RT-qPCR in leaves treated with 100 nM flg22 or water for 4 hours. (C) Callose deposition upon H₂O or flg22 (100 nM) treatment in WT and rdr6-15 leaves blade at stage 7. Values are average ± se (standard error) with n = 25 to 30. (D) Bacterial growth in five- to six-week-old plants (WT or rdr6) 4 days after being sprayed (10⁸ CFU mL⁻¹) with Pto DC3000. Values are average ± se of four leaf discs (n = 8). Wilcoxon test was performed to determine the significant differences between rdr6 and WT plants. Asterisk “**” indicates statistically significant differences (P<0.01). Experiments were performed in two independent biological replicates with similar results.