Figure 3. RDR6-dependent siRNAs negatively regulate a subset of CNL transcripts both constitutively and during flg22 elicitation.

(A) The transcript levels of At1g12220 (RPS5), At1g51480 (RSG1) and At5g43730 (RSG2) were detected by RT-qPCR in untreated seedlings. Results of expression represent the ratio rdr6/WT. (B) Transcript levels of RPS5, RSG1 and RSG2 by RT-qPCR in seedling treated or not with flg22 (100 nM) at different time-points. Results represent the ratio of the values between samples treated with flg22 relative to H₂O (mock) for rdr6 and WT seedlings. Expression levels are always normalized to the same internal controls At2g36060, At4g29130, and At5g13440. Error bars indicate standard deviation from technical repeats. These experiments were performed in two biological replicates with similar results.