Table 2. Transcription and translation coordinates, timing of expression, and function of late lytic ORFs.

Gene	Str	TSS-PolyA	Start-Stop	Ann	Variant	Timing	Function	Ref
ORF8	+	8656–17054	8680–11217	P		48 h–72 h	Glycoprotein B	[113]
ORF9	+	N/A-17054	11344–14382	P		48 h–72 h	DNA Polymerase	[114]
ORF10U	+	N/A-17054	14500–15756	M		48 h–72 h	Regulator of Interferon Function	[115]
ORFK3A	−	21099–18575	19128–18589	P		48 h	Immune modulator	[94], [95]
K5/6-AS	+	N/A-29690	N/A	M		48 h	N/A	[28]
ORF17	−	32300–30563	32243–30639	P		48 h	Protease	[116]
ORF21U	+	35120–39147	35201–36943	M		48 h–72 h	Thymidine kinase	[117]
ORF22	+	N/A-39147	36931–39123	P		48 h–72 h	Glycoprotein H	[108]
ORF23	−	N/A-39048	N/A	N/A		48 h–72 h	Glycoprotein (predicted)	[118]
ORF24	−	N/A-39048	N/A	N/A		48 h–72 h	Essential for replicationMHV68	[119]
ORF25	+	42378–48596	42595–46725	P		48 h–72 h	Major capsid protein	[120]
ORF26	+	46724–48596	46751–47668	P		48 h–72 h	Minor capsid protein	[120]
ORF27	+	N/A-48596	47690–48562	P		48 h–72 h	GlycoproteinMHV68	[121]
ORF28U	+	48748–53916	48808–49116	P		48 h–72 h	BDLF3 EBV homolog	[122]
ORF29	−	N/A	N/A	N/A		72 h	packaging protein	[123]
ORF30U	+	N/A-53916	50440–50673	M		48 h–72 h	Late gene regulationMHV68	[124]
ORF31	+	N/A-53916	50580–51254	M		48 h–72 h	Nuclear and cytoplasmicMHV68	[124]
ORF32	+	N/A-53916	N/A-52585	M	Internal	48 h–72 h	Tegument protein	[124]
ORF33	+	52553–53916	52578–53582	P		48 h–72 h	Tegument proteinMHV68	[125]
ORF40/41	+	N/A-62375	60124–62260	P		48 h–72 h	Helicase-Primase	[126]
ORF42	−	63108–62232	63088–62252	P		48 h–72 h	Tegument protein	[127]
ORF43	−	N/A-62232	64769–62952	M	AS-ORF	48 h–72 h	Portal protein (capsid)	[128]
ORF44	+	N/A-67134	64708–67074	M		48 h–72 h	Helicase	[114]
ORF45.1		68952–67140	multiple	P		48 h	N/A
ORFK8.1	+	75716–76554	75731–76511	P	Sp. Var	48 h	glycoprotein	[24], [25]
ORF52	−	77063–76523	77013–76618	P		48 h–72 h	Tegument protein	[129]
ORF53	−	77513–76523	77481–77149	P		48 h–72 h	Glycoprotein N	[105]
ORF54	+	N/A-78588	77483–78439	M	Alt. Start	48 h–72 h	dUTPase/Immunmodulator	[53]
ORF55U	−	79525–78521	79264–78581	P		48 h–72 h	Tegument protein
ORF56	+	N/A-83490	N/A	N/A		48 h–72 h	DNA replication	[61]
ORFK9	−	N/A-83605	N/A	N/A		48 h–72 h	vIRF1	[130]
ORFK10	−	N/A-85823	N/A	N/A		48 h–72 h	vIRF4	[130]
ORFK10.5	−	N/A-89190	N/A	N/A		48 h–72 h	vIRF3	[130]
ORFK11	−	N/A-91573	N/A	N/A		48 h–72 h	vIRF2	[130]
ORF62	−	101148–94287	101013–100018	P	Alt. Start	72 h	N/A	[61]
ORF65U	−	112340–111650	112262–111750	P		48 h–72 h	capsid	[131]
ORF66	−	N/A-111650	N/A	N/A		48 h–72 h	capsid	[132]
ORF67	−	N/A-111650	114327–113512	M		48 h–72 h	Nuclear egress complex	[133]
ORF67.5	−	N/A-111650	114624–114382	P		48 h–72 h	N/A
ORF68	+	N/A-117240	multiple	P	Internal	48 h–72 h	Glycoprotein	[134]
ORF69	+	116029–117240	116257–117165	P		48 h–72 h	BRLF2 Nuclear egress	[133], [134]
ORFK14	+	128171–130870	128210–129256	P		24 h–48 h	vOX2	[135]
ORF74	+	128171–130870	N/A	N/A		24 h–48 h	vGPCR	[135]
ORF75U	−	134923–130850	134766–130876	P		48 h–72 h	FGARAT	[136]

Transcription and translation coordinates for the primary viral ORFs were annotated from in silico predictions and/or by manually curating mRNA-seq and Ribo-seq data. Timing was determined according to the time of transcription. Functions were gathered from the literature. U: uORF in leader region; Str: Strand; TSS: transcription start site; N/A, not determined in silico or by manual annotation; Ann: Annotation; P: predicted; M: manual annotation; Sp. Var: ORF with splice variant, Alt. Start: ORF with alternative starts; Internal: ORF with internal ORFs; AS-ORF: Antisense ORFs; MHV68: Function annotated in MHV68.