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. 2014 Jan 16;10(1):e1003878. doi: 10.1371/journal.ppat.1003878

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© 2014 Patocka et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The amino acid sequences of known and predicted serotonin receptors were aligned using the ClustalW method and a Neighbor-Joining Best Tree was constructed from the alignment. The alignment included representative examples of all major classes of serotonergic GPCRs but only three clades are shown for simplicity. These include type 2 serotonin receptors (5HT2), type 7 (5HT7) and structurally related type 1 and type 5 receptors (5HT1/5). The outgroup in the alignment (not shown) was a distantly related rat metabotropic glutamate receptor (NP_058707). The tree was rooted to the outgroup and was tested by bootstrap analysis with 1000 iterations. The length of the branches is proportional to the genetic distance between sequences. Sequences were all obtained from the National Center for Biotechnology Information (NCBI). S. mansoni sequences are designated by their S. mansoni GeneDB “smp”designation number and are marked by arrows. Accession numbers for the remaining sequences are as follows: 5HT2_Drosophila (NP_730859); 5HT2_Lymnae (AAC16969.1), 5HT2(ser-1)_C. elegans (AAC15778); 5HT2B_Xenopus (NP_001082744); 5HT2A_human (NP_000612); 5HT2A_mouse (NP_766400); 5HT2B_human (NP_000858); 5HT2C_human (NP_000859); 5HT5B_mouse (NP_034613.2); 5HT5A_human (NP_076917.1); 5HT1_Helisoma (AAQ95277.1); 5HT1A_Xenopus (NP_001079299.1); 5HT1A_human (NP_000515.2); 5HT1A_chicken (NP_001163999.1); 5HT1D_dog (P11614); 5HT1A_pufferfish (O42385); 5HT1_Aplysia (NP_001191550); 5HT1(ser-4)_C. elegans (NP_497452); 5HT_Haemonchus (AAO45883); 5HT7_Xenopus (NP_001079253.1); 5HT7_human (NP_062873.1); 5HT7_chicken (NP_001167605.1) 5HT7_Helisoma (AAQ84306.1); 5HT7_Meleagris (XP_003206102); 5HT7_zebrafish (XP_690599); 5HT7_Clonorchis (GAA29051); 5HT7_Aedes (AF296125); 5HT7 (ser-7)_C. elegans (NP_741730); 5HT_Dugesia.1 (BAA22404); 5HT_Dugesia.4 (BAA22403); 5HT7_Dugesia (BAI44327.1); 5HT4_chicken (XP_414481.2); 5HT4_human (NP_000861.1); 5HT6_chicken (NP_001166911.1); 5HT6_human (NP_000862.1); 5HT6_mouse (NP_067333.1); ratmGlu.8393487; mGluR1_rat (NP_058707).