FIGURE 9.

Deletion of uge3 and uge5 renders the resulting Δuge3Δuge5 double mutant deficient in Galf and galactosaminogalactan. A, indicated strains were grown for the indicated time periods in Brian medium using either glucose or galactose as the sole carbon source. Brightfield images at a magnification of 200x are shown. Glucose is abbreviated as Glc, and galactose as Gal. B, indicated strains were grown for 2 days on either glucose or galactose based Brian medium agar plates. C, relative Galf detection by ELISA in culture supernatant after 72 h growth in Brian medium of the indicated strains. D, galactosaminogalactan production after 72 h growth in Brian medium of the indicated strains. *, significant reduction compared with Af293 wild type and analysis of variance with pairwise comparison, p < 0.05.