FIGURE 3.

Results of 2PPM imaging of Gαi/o-FP constructs. Shown is a summary of data from 2PPM experiments on Gαi/o-FP constructs of the GAP43-CFP-Gαi/o (A–D) and Gαi/o-L91-YFP (E–H) designs. The graphs show log₂(r_max) and r_max of the Gαi/o-FP constructs transfected alone (white bars); cotransfected with Gβ1 and Gγ2 subunits (light gray bars); cotransfected with Gβ1, Gγ2, and α2AR-FP constructs and activated by addition of 1 μm NE (medium gray bars), constitutively active mutants of Gαi/o-FP constructs transfected alone (dark gray bars), and cotransfected with Gβ1 and Gγ2 subunits (black bars). Error bars represent mean + S.E.