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DCIS stem cells possess enhanced migratory capacity. We isolated CD49f+/CD24− stem-like cells, which were enriched in ALDH1 activity, from MCF10DCIS cells, and we performed transwell migration assays. After 24 h of migrating toward a chemoattractant (10% FBS), we stained migrated cells with 1% crystal violet and visualized with light microscopy. Five random fields were counted (n = 3).
