FIGURE 6.

SFN treatment targets DCIS stem-like cells. A, SFN treatment (10 μm) reduces progenitor colony formation. Colonies were stained with crystal violet, and colonies were quantified by light microscopy. B, SFN treatment reduced mammosphere formation of DCIS stem-like cells. Isolated CD44-high/CD24-low stem-like cells were culture in mammosphere conditions with or without SFN treatment (0, 10, 20 μm), and after 7 days, spheres > 100 μm were quantified by light microscopy. C, SFN treatment reduces ALDH1 expression. Western blotting was used to quantify ALDH1 expression following SFN treatment (10 μm). β-Actin was used as a loading control.