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A, electron micrograph from a wild-type mouse islet showing a β-cell containing secretory granules (g). Mitochondria (m) and nucleus (Nu) are indicated. B, higher magnification of granular vesicles. Mice (6–10 weeks old) were perfusion-fixed with 1.5% formaldehyde and 2.5% glutaraldehyde in 0.1 m sodium phosphate buffer, pH 7.4. Scale bar, 2 μm.
