FIGURE 2.

A, schematic representation of different vector constructs. The pBADA2 vector encodes the target protein followed by a tobacco etch virus (TEV) protease cleavage site and a deca-His tag at the C terminus. The pBAD-CM vector is modified in a way in which the target gene can be cloned two times, one time separately with an added hexa-His tag coding sequence and another time with an added Strep-I tag coding sequence. B, oligomerization study of STM2200 using a pBAD-CM vector. The Western blot of co-purified proteins was developed separately with an anti-His antibody and a streptavidin, both coupled to alkaline phosphatase. Lane M, marker; lane 1, His tag-purified protein; lane 2, Strep-I tag-purified protein.