FIGURE 7.

TNFα (10 ng/ml) has no effect on K⁺ current at external pH 6. 4 and pH 7.4 in tsA-201 cells transfected with truncated mutant hTASK3. (a) Traces of current obtained using the voltage protocol on cells incubated in an external solution of pH 6.4 or pH 7.4 treated with TNFα compare with untreated cells. b, current-voltage plot of hTASK3 current density in tsA-201 cells untreated or treated with TNFα for 2 h and incubated in an external solution of pH 6.4 and pH 7.4. c, histogram of current density (mean ± S.E. (error bars)) in control cells and cells treated with TNFα at an external pH of 6.4 or 7.4 (one-way ANOVA; NS, p > 0.05). Significant inhibition of current induced by pH 6.4 is compared with pH 7.4 for control and TNFα-treated cells (one-way ANOVA; ++, p ≤ 0.01)