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. 2013 Dec 4;289(3):1388–1401. doi: 10.1074/jbc.M113.500033

FIGURE 8.

FIGURE 8.

TNFα enhances K+ current via ROS-ASK1 pathway in tsA-201 cells transfected with wild-type hTASK3. a, current-voltage plot of hTASK3 current density in tsA-201 cells co-transfected with either ASK1-wt gene or ASK1-KM gene and treated with TNFα for 2 h. b, histogram of current density (mean ± S.E. (error bars)) in control cells and cells co-transfected with either ASK1-wt gene or ASK1-KM gene, treated with TNFα. c, reactive oxygen species produced in comparison with control (100%) after treatment in TNFα in cells co-transfected with either ASK1-wt gene, ASK1-KM gene or without co-transfection. d, activity of ASK1 protein in control cells treated or untreated with TNFα and in cells co-transfected with either ASK1-wt gene or ASK1-KM gene treated with TNFα (one-way ANOVA; *, p < 0.05; **, p < 0.01; NS, p > 0.05). e, Western blot analyses of ASK1 protein levels.