TABLE 2.
The wild-type and mutated sequence of the FGF16 promoter and the sequence and Tm of the respective oligonucleotide primers used in in-vitro mutagenesis is mentioned
| Clone name | Forward primer (5′-3′) | Reverse primer (5′-3′) | Tm |
|---|---|---|---|
| °C | |||
| Mut 1 | GGAAGGGAGATCTGTGCGGGCCTC | AATGCAATTCTTAAGGGAATATTCTATGTAATC | 55 |
| Mut2 | GGAAGGGAGATCTGTGCGGGCCTC | CCAAAGTGTTGGGACAGGCATGCACCAC | 55 |
| Mut3 | GGAAGGGAGATCTGTGCGGGCCTC | GTCAACTAGATTCCTCTGGTATACCTGCCTGTAATCAC | 55 |
| Mut4 | GGAAGGGAGATCTGTGCGGGCCTC | GAGGCCAGCCCTGTGTGCAAAATGCCTGATGAAACC | 55 |