TABLE 4.

The sequence, respective amplicon size and T_m of the oligonucleotide primers used in Q-PCR

Gene Name	Forward primer (5′-3′)	Reverse primer (5′-3′)	Amplicon size	Tm
			bp	°C
PCNA	GAGGCCTGCTGGGATATTAGC	GGGTGAGCTGCACCAAAGAG	70	59
FGF16	CACGGCTTCTCCTCGTCTCT	AGGCGCTCGTTCAGGAAA	68	58
CCND1	TATTGCGCTGCTACCGTTGA	CCAATAGCAGCAAACAATGTGAAA	90	60
18S rRNA	GATTCCGTGGGTGGTGGTGC	AAGAAGTTGGGGGACGCCGA	134	60
POLR2A	TGGACCCACCGGCATGTTCT	GCCCCTGGGGTCATTCCACT	141	60
C-MYC	TCAAGAGGCGAACACACA AC	GGCCTTTTCATTGTTTTCCA	110	60
PITX2	CGCGAAGAAATCGCTGTGT	CGACGATTCTTGAACCAAACC	78	58
AXIN2	CGGGAGCCACACCCTTCT	TGGACACCTGCCAGTTTCTTT	80	60
CTNNB1	CCTTTGTCCCGCAAATCATG	CGTACGGCGCTGGGTATC	100	60
PITX2A	CGGCGTGTGTGCAATTAGAG	CGCCCACGTCCTCATTCT	80	60
PITX2B	GGCCGTTGAATGTCTCTTCT	CCTCTTTTCGGCTCTCAGG	80	60
PITX2C	CCGTCTCCTCATCTTCCTGT	TGTGCTTGGAGGAGTCCAG	80	60
BCL2	CATGTGTGTGGAGAGCGTCAA	GCCGGTTCAGGTACTCAGTCA	83	60
CDH1	GTCACTGACACCAACGATAATCT	TTTCAGTGTGGTGATTACGACGTA	100	60
MMP2	TGATCTTGACCAGAATACCATCGA	GGCTTGCGAGGGAAGAAGTT	90	60
MMP9	ACCTCGAACTTTGACAGCGAC	GAGGAATGATCTAAGCCCAGC	113	60
SNAI1	TCGGAAGCCTAACTACAGCGA	AGATGAGCATTGGCAGCGAG	140	60