FIGURE 2.

Identification and characterization of native MF6p/FhHDM-1. A and B, the immunopurified MF6p/FhHDM-1 complexes were separated by RP-HPLC while monitoring the absorbance at 210 and 402 nm, and the m/z values of the peaks were determined by MALDI-TOF MS to be 616.12, suggestive of a heme B group, and 7,832.5, corresponding to MF6p/FhHDM-1. AU, absorbance units. C, the MS/MS sequencing of the tryptic peptide encoding the amino acid residues 63–86 enabled identification of two ESTs, one of which was used to clone the entire cDNA sequence coding for MF6p/FhHDM-1. The full-length amino acid sequence of MF6p/FhHDM-1 deduced from the cloned cDNA sequence (CCA61804.1) is shown. The predicted N-terminal signal peptide is underlined.