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. 2013 Nov 26;289(3):1441–1456. doi: 10.1074/jbc.M113.499517

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FIGURE 5. — MF6p/FhHDM-1 is secreted through the tegument and is present in F. hepatica eggs. A, overall view of a frontal section of an F. hepatica adult stained with Wheatley's trichrome. Dorsal (B) and ventral (C) imprints of tegumental antigens from freshly killed adult flukes were revealed with mAb MF6. Strong staining of the whole tegument surface and no staining of the genital pore (Gp) or oral sucker (Os) was observed. Ca, cecum; Ov, ovary; Te, testis; Vs, ventral sucker. D, WB of soluble antigens from F. hepatica mature eggs (photograph) revealed with mAb MF6 (lane 1), showing the presence of MF6p/FhHDM-1. F. hepatica SAs were used as a positive control (lane 2). The negative control was revealed with mAb MM3 (lane 3).