FIGURE 9.
sMF6p/FhHDM-1 inhibits hemin peroxidase-like activity. Hemin alone (23 μm) and hemin with sMF6p/FhHDM-1 (at increasing protein/hemin molar ratios) were mixed with 100 μl of a commercial solution of TMB (1 mm TMB, 3 mm H2O2) adjusted to pH 6; TMB oxidation was monitored at 650 nm for 30 min. A, the absorbance at 650 nm of hemin alone (squares) and hemin with sMF6p/FhHDM-1 (at a 5:1 ratio; circles) was plotted against time. B, inhibition percentage of TMB oxidation by hemin as a function of the protein/hemin molar ratio.