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Additive effect of glucose and glutamine on NADPH availability at low but not at high concentrations. NADPH availability was measured from the Fex-340/Fex-380 ratio of rod outer segment fluorescence (emission, >420 nm), 60 min after bleaching. Low concentrations were 50 μm glucose and 50 μm glutamine; high concentrations were 5 mm glucose and 0.5 mm glutamine. Cell numbers are shown within each column. The error bars represent standard errors. Experiments were performed at 37 °C.
