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Glutamine reverses the suppression of NADPH generation by 4-CIN. NADPH generation was measured from its availability as the Fex-340/Fex-380 ratio of rod outer segment fluorescence (emission, >420 nm), 60 min after bleaching. Glucose and glutamine concentrations were 5.0 and 0.5 mm, respectively. Oligomycin and 4-CIN concentrations were 5 μm and 1.0 mm, respectively. Cell numbers are shown within each column. Error bars represent standard errors. Experiments were performed at 37 °C.
