FIGURE 5.

Overexpression of DGKζ enhances the serum-induced activation of mTOR signaling in a kinase activity-dependent manner. C2C12 myoblasts were co-transfected with plasmid DNA encoding GFP, wild type DGKζ (HA-WT-DGKζ), or kinase dead DGKζ (HA-KD-DGKζ) and GST-p70. The following day the cells were serum-starved overnight and then stimulated with 20% fetal bovine serum (Serum +) for 30 min. The cells were collected and then subjected to Western blot analysis for the indicated proteins. The phospho (P):total (T) ratios of GST-p70 were then expressed as a percentage of the values obtained in the GFP non-stimulated (control, Serum −) samples. Values are presented as the mean ± S.E. and were obtained from five independent experiments (n = 5–9 per group). *, significantly different from the plasmid-matched control group. †, significantly different from the stimulation-matched GFP group; p ≤ 0.05.