FIGURE 3.

Normal T-cell development in Cmah^−/− mice. A, splenocytes of wild-type and Cmah^−/− mice were co-stained with anti-CD4 and anti-CD8 antibodies and analyzed by flow cytometry. B, splenic T cells of wild-type and Cmah^−/− mice were stained with anti-TCR antibody and analyzed by flow cytometry. Black solid lines, results obtained when anti-TCR antibody was used; gray dashed lines, results from unstained controls. C, splenic T cells of wild-type and Cmah^−/− mice were co-stained with anti-CD4 and anti-CD25 antibodies. The percentages of CD4⁺CD25⁺ regulatory T cells are indicated. Data are representative of several independent experiments that yielded similar results.