Skip to main content
. 2013 Nov 27;289(3):1604–1616. doi: 10.1074/jbc.M113.520254

FIGURE 1.

FIGURE 1.

Gp78 depletion restricts RNA virus replication. A, decreased CVB and PV replication by high-throughput RNAi screening in HBMEC transfected with Gp78 siRNAs (siGp78) compared with an siRNA targeting a gene within the library that had no effect on viral replication (si-No Effect). VP1 staining is shown in green, and DAPI-stained nuclei are shown in blue. White text at bottom left denotes the level of infection (%). B, decreased VSV-GFP replication in HT1080 cells transfected with siGp78 compared with control siRNA (siCON), as assessed by immunofluorescence microscopy at 8 h post-infection. VSV-GFP is shown in green, and DAPI-stained nuclei are shown in blue. C, decreased VSV-GFP and CVB replication in HT1080 cells transfected with siGP78 compared with control siRNA (siCON), as assessed by immunofluorescence microscopy at 8 h (VSV-GFP) or 16 h (CVB) post-infection. D, level of Gp78 expression in HT1080 cells transfected with siGp78 compared with a control siRNA, as assessed by RT-qPCR 48 h post-transfection. All data are representative of at least three independent experiments, and data in C and D are presented as mean ± S.D. (*, p < 0.01).