FIGURE 1.

5′ serial truncation and internal deletion constructs of mouse Cnn2 gene promoter. A pcDNA3.1(+)/CAT plasmid was modified by replacing the CMV promoter with a 3.77-kb promoter segment of mouse h2-calponin gene. Six 5′-truncation constructs and an internal deletion construct were derived from this recombinant plasmid. The −2.12- (−2,115 to +6) and −0.61-kb (−611 to +6) constructs were generated using restriction enzyme digestion. The −1.57- (−1,572 to +6), −1.38- (−1,380 to +6), −1.24- (−1,244 to +6), and −1.00-kb (−1,002 to +6) constructs were generated with PCR using customer-designed primers (black arrowheads). The −1,572 to −1,380 internal deletion was made in the −2.12-kb construct with recombinant PCR using deletion primers containing complementary 5′ sequences (gray arrowheads).