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. 2013 Nov 27;289(3):1617–1628. doi: 10.1074/jbc.M113.498147

FIGURE 2.

FIGURE 2.

Substrate stiffness-regulated expression of endogenous h2-calponin in HEK293, NIH/3T3, and C2C12 cells. The SDS-PAGE gels and RAH2 Western blots detected significant levels of endogenous h2-calponin in HEK293 epithelial cells, NIH/3T3 fibroblasts, and C2C12 myoblasts. Normalized to the level of actin, the levels of h2-calponin in the three cell lines were significantly decreased when cultured on soft gel substrate (5%, acrylamide:bisacrylamide ratio = 500:3, Ef = ∼1 kilopascal) versus that in cells cultured on hard gel substrate (16%, acrylamide:bisacrylamide ratio = 19:1, Ef = ∼75 kilopascal).