FIGURE 7.

Substrate anchorage-dependent expression of h2-calponin in NIH/3T3 cells is counter-correlated with the level of HES-1. NIH/3T3 cells were cultured in plastic dishes steadily as an adherent monolayer or under continuous vibration that prevented the cells from anchoring on the dish for 3 days (26). Total cellular protein was examined with SDS-PAGE (14% gel with acrylamide:bisacrylamide ratio of 180:1) (A) and Western blotting using anti-h2-calponin polyclonal antibody RAH2 (B) and anti-HES-1 monoclonal antibody 7H11 (C). Normalized by the level of actin, the results showed that floating cells under low tension expressed lower level of h2-calponin as expected, which corresponded to a higher level of HES-1. **, p < 0.001.