FIGURE 2.

C. albicans Glx3 is a monomeric member of the Hsp31 clade of the DJ-1 superfamily. A, ribbon diagram of Glx3 is shown with β-strands numbered and in red and α-helices lettered in blue. The N and C termini are labeled. Active site residues are shown in bond representation. B shows a close-up view lattice contract that represents the largest intermolecular interface between Glx3 proteins in the crystal. The two proteins are colored pale blue and gold, with primes on residues from the other chain. 2mF_o − DF_c electron density is shown contoured at 1σ and colored blue. The carboxylic acid-rich contact region between two proteins and the close 2.44-Å hydrogen bond between Glu¹³ and Glu⁴⁸ requires low pH to be stable and would not form under physiological conditions. Therefore, there are no intermolecular contacts of probable physiological relevance in the Glx3 crystal.