TABLE 1.

Primers used in this study

Sequence	Name	Reference	Purpose
5′-TCC GAG GGG GGT ACC TCC TTC-3′	KpnI	This study	Glx3 knockout
5′-TTG AAA CAC TCG AGG AAG GGG-3′	XhoI	This study	Glx3 knockout
5′-GGG CAT ACA AAA GCC GCG GAT TTA-3′	SacI	This study	Glx3 knockout
5′-AAC AAA ATT AAT TGA GCT CTC TTG-3′	SacII	This study	Glx3 knockout
5′-TAT TGT TGA TGA ATC GAA GAA AAC-3′	Upstream check	This study	Southern blot
5′-TGT TTA GTA GAT CTT TCT AGA TCT-3′	Downstream check	This study	Southern blot
5′-TAA AAA TAT CAT ATG GTG AAA GTT TTA CTC-3′	NdeI	This study	Glx3 insertion in pET15b
5′-TAA TTT CTC GAG TTA ATT ACA TTC AAA AGG-3′	XhoI	This study	Glx3 insertion in pET15b
5′-CTT TCT GCT GTT AGT CAT GGT CCT GCC AT-3′	Forward C136S	This study	Glx3 (C136S) point mutation
5′-ATG GCA GGA CCA TAA ACA GCA GAA AC-3′	Reverse C136S	This study	Glx3 (C136S) point mutation
5′-GTT TGT TTT GGT CCT GCC ATT TTT-3′	Forward H137F	This study	Glx3 (H137F) point mutation
5′-AAA AAT GGC AGG ACC AAA ACA AAC-3′	Reverse H137F	This study	Glx3 (H137F) point mutation