TABLE 3.

Comparative glyoxalase-specific activities of selected DJ-1 superfamily proteins

	Human DJ-1	Sz. pombe DJ-1	S. cerevisiae YDR533C	C. albicans Glx3	C. albicans C136S Glx3	C. albicans H137F Glx3
Methylglyoxal consumption (μmol/min/mg enzyme)	1.0 ± 0.3a	2.4 ± 0.2	6.4 ± 0.1	11.5 ± 0.1	0.2 ± 0.1b	1.7 ± 0.1
d-Lactate production (μmol/min/mg enzyme)	NDc	ND	10.5 ± 1.0	7.0 ± 1.0	ND	ND

^a Values shown are the average of triplicate experiments ± S.D. using purified proteins as expressed in E. coli. The methylglyoxal assay was performed at 30 °C and the d-lactate assay at 37 °C.

^b This value is near the noise level of the measurement and is consistent with no significant activity.

^c Data not detected with an estimated detection limit of ∼0.2 μmol/min/mg enzyme for NADH production.