TABLE 1.

Mutations to substitute residues from the ATII-LCL MerA to their corresponding amino acids in the soil enzyme

Mutation	From	To
M1	E15 and E16 (E15/E16)	A15 and A16 (A15/A16)
M4	E133 and E134 (E133/E134)	G133 and G34 (G133/G134)
M15 (box1)	KPAR432–435 (432KPAR435)	LDLT432–435 (432LDLT435)
M16 (box2)	KVGKFP465–470 (465KVGKFP470)	DSRTLT465–470 (465DSRTLT470)
M15/M16 box1/box2	432KPAR435/465KVGKFP470	432LDLT435/465DSRTLT470
M17	E515 and E516 (E515/E516)	A515 and A516 (A515/A516)
M18	E545 and E546 (E545/E546)	A545 and A546 (A545/A546)