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HARE-mediated NF-κB activation by Hep, DS, or AcLDL is not due to HA contamination. HARE cells were incubated for 4 h with samples (20 nm) of 51-kDa HA, Hep, DS, or AcLDL, which had been treated with or without S. hyalurolyticus hyaluronidase, and processed for relative luciferase activities as described under “Experimental Procedures” and Fig. 1 (n = 9): ***, p < 0.001.
