Mitochondrial morphology is not affected by unremodeled CL. Mitochondria from the indicated strains derived from GA74 (A–E) and W303 (F–J) yeast were analyzed by transmission electron microscope. A and F, representative micrographs from the indicated strains. m, mitochondria; n, the nucleus. Bars, 0.5 μm. B and G, quantification of cristae length per mitochondrion. C and H, quantification of outer membrane length per mitochondrion. D and I, quantification of aberrant mitochondria for each strain, defined as the appearance of exaggerated cristae >0.5 μm in length. The number of mitochondria analyzed is indicated for each strain. E and J, examples of mitochondria with exaggerated cristae. Bars, 0.5 μm. *, p < 0.05; **, p ≤ 0.01; ***, p ≤ 0.001.