FIGURE 5.
OXPHOS function is not affected by unremodeled CL. A, mitochondria were solubilized with digitonin, separated by blue native-PAGE (top panels), and immunoblotted for Cyt1p (complex III), Cox2p (complex IV), Atp2p (complex V), and Aac2p. Bottom panels are immunoblots following SDS-PAGE, which serve as loading controls. B and C, respiration measured in the presence of 2 mm NADH. n = 6–9. B, phosphate/oxygen ratios. C, respiratory control ratios. D, representative TMRM time traces of mitochondria isolated from the indicated yeast strains following the addition of 2 mm NADH (to establish the Δψm) and two sequential additions of 45 μm ADP to induce phosphorylation cycles, manifest as transient depolarizations. E, average times required for the re-establishment of maximal (state 4) Δψm following ADP addition for the yeast strains indicated. *, p < 0.05; ***, p < 0.001.