TABLE 6.

Determination of lisinopril binding for all constructs

The K_d values (nm) for lisinopril binding at 0 and 20 mm for the C-domain, the N-domain and a number of C-domain mutant constructs. Constants were determined using an ITC assay at 0 and 20 mm NaCl in 50 mm TAPSO (pH 7.5) and 10 μm ZnSO₄. The S.D. value was calculated using the non-linear regression fitting function provided.

Construct	[NaCl]	Kd
	mm	nm
N-domain	0	47.80 ± 4.94
	20	4.61 ± 0.63
C-domain	0	45.50 ± 4.24
	20	13.50 ± 2.06
R186H	0	132.00 ± 6.72
	20	4.42 ± 0.50
E403R	0	2.67 ± 0.31
	20	6.76 ± 0.65
R522Q	0	22.00 ± 3.31
	20	15.90 ± 2.09
R522K	0	35.30 ± 2.85
	20	26.30 ± 1.86