FIGURE 3.

WA treatment decreases the protein levels of α- and β-tubulin in human breast cancer cells. A, representative immunoblots for α- and β-tubulin proteins using lysates from MCF-7, SUM159, and SK-BR-3 human breast cancer cells treated with DMSO or the indicated doses of WA for 8, 16, or 24 h. The change in tubulin protein levels relative to the corresponding DMSO control is shown on top of the bands. B, quantitation of tubulin protein expression. Results are shown as means ± S.D. (n = 3–5). *, significantly different (p < 0.05) compared with the corresponding DMSO control by one-way analysis of variance, followed by Dunnett's test. C, immunoblotting for β-tubulin using lysates from MCF-7 cells treated with DMSO or the indicated doses of WE or WLA for 8, 16, or 24 h. D, quantitation of the effects of WE and WLA treatments on β-tubulin protein expression in MCF-7 cells. Results shown are means ± S.D. (n = 3–4).