FIGURE 5.

WA disrupts microtubule organization and causes aberrant mitotic spindle morphology. A, confocal images (×63 objective magnification (oil)) of α- and β-tubulin expression in interphase MCF-7, SUM159, and SK-BR-3 human breast cancer cells treated for 24 h with DMSO or 2 μm WA. Scale bars = 5 μm for MCF-7 and SUM159 cells and 20 μm for SK-BR-3 cells. B, confocal images of representative mitotic cells in MCF-7 cultures treated for 24 h with DMSO or 2 μm WA. Mitotic spindles were imaged using anti-α-tubulin or anti-β-tubulin antibody (red), and chromosomes were stained with DAPI (blue). Scale bars = 5 μm.