FIGURE 6.

MCF-10A cells are relatively less sensitive to WA-mediated mitotic arrest compared with breast cancer cells. A, representative flow histograms depicting cell cycle distribution in MCF-10A cells after a 8- or 24-h treatment with DMSO or 2 μm WA. Mitotic fraction are identified by arrows. B, quantitation of G₂ and mitotic cells from data shown in A. Results shown are means ± S.D. (n = 3). *, statistically significant (p < 0.05) compared with the control by Student's t test. C, quantitation of the effect of WA treatment (8, 16, or 24 h) on the protein levels of α- and β-tubulin determined by Western blotting. Results shown are means ± S.D. (n = 3–5). *, significantly different (p < 0.05) compared with the vehicle-treated control by one-way analysis of variance with Dunnett's adjustment. D, confocal images (×63 objective magnification (oil)) depicting the effect of WA treatment (24 h) on α- and β-tubulin protein expression in interphase MCF-10A cells. Scale bars = 5 μm. Results were comparable in replicate experiments.