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Sequence-specific quantitation of the modified and unmodified tryptic peptides (NMMAACDPR) in MCF-7 cells treated with WA and DMSO. A, tandem mass spectrum of peptide NMM#AAC(WA)DPR with sequence-specific product ions (b6 and y4), confirming the presence of the Cys303-WA adduct. B, tandem mass spectrum of the unmodified NMMAACDPR peptide in samples derived from MCF-7 cells incubated with DMSO. C, quantitation of the b7+ product ion in MCF-7 cells treated with WA and DMSO.
