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. 2013 Dec 3;289(3):1866–1875. doi: 10.1074/jbc.M113.505578

FIGURE 3.

FIGURE 3.

Expression of AhR and CYP1A1 in MEF cells from wt and Rel null mice. A, expression of AhR mRNA; B, CYP1A1 mRNA; C, ARNT mRNA in MEF cells were analyzed using real-time PCR. MEF cells derived from wild type (wt), RelA-deficient mice (RelA−/−), RelB-deficient mice (RelB−/−), and AhR-deficient mice (Ahr−/−) were treated with 1 nm TCDD, 0.1 μg/ml LPS, or 0.1% DMSO (Ctrl; control) for 6 h. D, induction of AhR and CYP1A1 is restored in MEF RelA−/− cells after transient transfection with a RelA. Cells were transiently transfected with a control or RelA expression plasmid (pRelA) for 24 h and treated with 1 nm TCDD, 0.1 μg/ml LPS, or 0.1% DMSO (Ctrl) for 6 h. E, Western blot analysis of AhR, CYP1A1, and RelA protein levels in WT and RelA−/− MEF. 25 μg of whole cell protein was loaded per lane. F, AhR and CYP1A1 protein levels were quantitated and normalized to actin. Values represent the mean ± S.D. of three independent experiments. A single asterisk indicates significantly different from control cells (p < 0.05). Values for AhR, ARNT, and CYP1A1 mRNA expression are normalized to the expression of β-actin. Values are the mean ± S.D. of three independent experiments. A single asterisk indicates significantly different from control cells (p < 0.05).