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. 2013 Dec 3;289(3):1866–1875. doi: 10.1074/jbc.M113.505578

FIGURE 4.

FIGURE 4.

LPS-specific effects on deletion and mutation constructs of the human AhR promoter. A, schematic illustration of the full-length promoter construct of the human AhR gene containing 5640 bp upstream of the transcriptional start site (indicated by an arrow) cloned into a luciferase (luc) reporter vector. Positions of the three putative NF-κB recognition sites are presented. B, effect of LPS on AhR deletion constructs. U937-derived DC were transiently transfected with pGL3-hAhRP, and the deletion constructs AhRΔ(−2510), AhRΔ(−881), or AhRΔ(−120). C, effect of LPS on AhR promoter constructs mutated in NF-κB-binding sites. U937-derived DC were transiently transfected with equimolar amounts of the designated deletion or mutation constructs and treated with 100 ng/ml LPS for 6 h. Mean + S.D. of three independent experiments are given. A single asterisk indicates significantly different from control (p < 0.05).