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. 2014 Jan 9;11(2):180–191. doi: 10.7150/ijms.6283

Figure 1.

Figure 1

The expression of RXFP1 protein in the total homogenate of lateral collateral ligament from ovariectomised rats treated with different sex-steroids: C- control; 0.2E - 0.2μg/kg estrogen; 2E - 2μg/kg estrogen, 20E - 20μg/kg estrogen; 50E -50μg/kg estrogen; P - 4mg progesterone; 125T - 125μg testosterone; 250T - 250μg testosterone, LCL-lateral collateral ligament. Data were expressed as mean ± SEM, n=6 per treatment group. * p<0.05 as compared to control, Shapiro-Wilk test, p= 0.319732. A dose-dependent increase in RXFP1 protein expression was observed following estrogen treatment. Progesterone caused the highest increase while testosterone caused a decrease in this isoform expression. The image was cut in order to arrange the bands in sequence with the label on the x-axis of the bar chart.