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. 2014 Jan 9;11(2):180–191. doi: 10.7150/ijms.6283

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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The expression of RXFP1 protein in the total homogenate of the patellar tendon from ovariectomised rats treated with different sex-steroids: C- control; 0.2E - 0.2μg/kg estrogen; 2E - 2μg/kg estrogen, 20E - 20μg/kg estrogen; 50E -50μg/kg estrogen; P - 4mg progesterone; 125T - 125μg testosterone; 250T - 250μg testosterone PT- patellar tendon. Data were expressed as mean ± SEM, n=6 per treatment group. * p<0.05 as compared to control, Shapiro-Wilk test, p= 0.282109. A marked increase in the expression of RXFP1 protein occurs following treatment with high doses of estrogen (20 & 50μg/kg). Meanwhile, progesterone treatment caused the highest increase while testosterone caused a decrease in RXFP1 protein expression in the tendon. The image was cut in order to arrange the bands in sequence with the label on the x-axis of the bar chart.