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. 2014 Jan 9;11(2):180–191. doi: 10.7150/ijms.6283

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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The expression of RXFP2 mRNA in the lateral collateral ligament from ovariectomised rats treated with different sex-steroids: C- control; 0.2E - 0.2μg/kg estrogen; 2E - 2μg/kg estrogen, 20E - 20μg/kg estrogen; 50E -50μg/kg estrogen; P - 4mg progesterone; 125T - 125μg testosterone; 250T - 250μg testosterone, LCL-lateral collateral ligament. Data were expressed as mean ± SEM, n=6 per treatment group. * p<0.05 as compared to control, Shapiro-Wilk test, p= 0.668854. Treatment with estrogen at doses exceeding 2μg/kg resulted in a dose-dependent increase in RXFP2 mRNA expression. Meanwhile, progesterone caused the highest increase while testosterone treatment resulted in a decrease in this isoform mRNA expression.