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. 2013 Nov 22;123(3):395–403. doi: 10.1182/blood-2013-08-520478

Figure 4.

Figure 4

Electron microscopy determines cellular location of 2bF8 transgene protein. Isolated platelets from (A-C) 2bF8LV-transduced xenotransplanted NSG recipients, (D-F) normal human individuals, and (G-I) untransplanted NSG control mice were immunostained for hFVIII and either endogenous human or mouse VWF. hFVIII was stained with mouse anti-human FVIII monoclonal antibody 103.3 and probed with goat anti-mouse colloidal gold probe (6 nm; some indicated by arrows). VWF was stained with rabbit anti-human VWF polyclonal antibody (Dako), which cross-reacts with mouse VWF, and were probed with goat anti-rabbit colloidal gold probe (10 nm; some indicated by arrowheads). The results show that hFVIII is stored together with human VWF in platelet alpha-granules of 2bF8LV-transduced human platelets (A-C).