Table 1.

Nonrestrictive linear amplification-mediated insertion sites of 2bF8 transgene determined by nrLAM-PCR

No.	Chromosome	Direction of transgene	Gene
1	1	↑	↓ DDI2 (DNA damage inducible protein 2) intron 1
2	1	↑	↑ ZSWIM5 (zinc finger, SWIM-domain-containing 5) intron 1
3	1	↑	↓ AHS1L (probable histone-lysine N-methyltransferase ASH1L) intron 14
4	3	↑	↓ CLASP2 (cytoplasmic linker-associated protein 2) intron 12
5	6	↑	Intergenic (located downstream of hypothetical protein LOC100288169 and upstream of DUSP22 [dual-specificity phosphatase 22])
6	7	↓	↑ DGKB (diacylglyceral kinase β isoform 1, 2) intron 2
7	7	↑	↑ GTF2IRD2 (general transcription factor II-I repeat domain-containing protein 2A) intron 2
8	7	↓	↓ PILRB (paired immunoglobulin-like type 2 receptor β precursor) intron 3
9	8	↑	Intergenic (located downstream of UNC5D [netrin receptor UNC5D precursor] and upstream of KCNUI [potassium channel subfamily U member])
10	11	↓	↓ RBM14-RBM41 (RBM14-RBM4 protein isoform 1, 2) intron 1
11	11	↓	↑ ARRB1 (β-arrestin-1 isoform A and B) intron 1
12	11	↓	↑ BSCL2 (Bernadinelli-Seip congenital lipidystrophy 2 [Seipin]) intron 5
13	11	↓	Intergenic (located downstream of LTBP3 [latent transforming growth factor β binding protein 3] and upstream of SSSCA1 [Sjogren syndrome/scleroderma autoantigen 1])
14	12	↓	Intergenic (located downstream of ATP2B1 [plasma membrane calcium-transporting ATPase 1 isoform 1a, 1b] and upstream of C12orf12 [hypothetical protein LOC196477])
15	14	↑	↓ SOCS4 (suppressor of cytokine signaling 4) exon 4 in 3′UT
16	14	↓	↑ GPR132 (G protein-coupled receptor 132) intron 1
17	16	↑	↑ CREBBP (Creb-binding protein) exon 30
18	16	↑	↑ KIAA0430 (Limkain b-1 isoform 1, 2, 3) intron 6
19	17	↓	↓ SUZ12 (suppressor of zeste 12 homolog [Drosophila]) intron 15

Arrows indicate the direction of the transgene or gene relative to the standard chromosomal numbering. The insertion sites listed in this table were from 5 NSG recipients.