FIG. 7.
In vivo serial imaging of luc+ bone marrow stromal cells after 20 Gy irradiation to the right hind leg. C57BL/6NHsd mice (n = 15) were irradiated with 20 Gy to the right hind leg. The mice were divided into three groups (5 mice/group) with the first group injected with 1 × 106 luc+ bone marrow stromal cells 24 h after irradiation, the second group injected on day 50 after irradiation and the third group injected 136 days after irradiation. The graphs (panels A–C) represent mean bioluminescence + SEM for each time point after irradiation and day after luc+ cell injection carried out according to published methods (44). A corresponding representative mouse from each group (panels D–F) is shown for the time points indicated in the graphs. In vivo imaging revealed no migration of 1 × 106 luc+ bone marrow stromal cells on days 1–15 (panels A and D); days 52–65 (panels B and E); or days 137–152 (panels C and F) after 20 Gy irradiation to the right hind leg.